Electrophoretic Analysis of Serum Proteins of Slaughtered Domestic Animals on Cellulose Acetate Membrane

Electrophoretic fractionation of serum glycoproteins on cellulose acetate.

In a procedure for the routine electrophoretic fractionation of serum glycoproteinson cellulose acetate, electrophoretic fractionation is followed by staining utilizing the periodicacid-Schiff reaction. The patterns are scannedwith a recording densitometer and simultaneouslyquantitated with an attached integrator. For 100 healthy blood donors,the meansand standarddeviationswere: albumin, 10.9±1...

Estimation of serum proteins by electrophoresis on cellulose acetate.

We have evaluated results for albumin obtained by a standard procedure for cellulose acetate electrophoresis of proteins in serum. The Ponceau S-stained .albumin and globulins were eluted and the albumin was calculated by the generally accepted formula [(albumin-bound dye absorbance/absorbance of total protein-bound dye) X total serum protein concn] and by the formula (absorbance of albumin-bou...

Rapid cellulose acetate electrophoresis. I. Serum proteins.

A method of electrophoresis utilizing cellulose acetate is described wherein quantitation of serum protein fractions by densitometry is accomplished within 2 hr. of initiation of the procedure. The densitometer was found to have a linear response to amido black dye. Albumin and y-globulin demonstrated equal dye-binding characteristics. Combined with the absence of albumin trailing, this has aff...

A study of the Electrophoretic Pattern of the serum Proteins

Parallel electrophoretic fractionation of alkaline phosphatase and serum protein on cellulose acetate strips: clinical evaluation.

We describe here a simple, time-saving method for separating alkaline phosphatases on cellulose acetate, with a parallel detection of serum protein pattern. The staining procedure is based on a coupling reaction between the reaction product of alpha-naphthol with Fast Red Violet LB. The enzymatic reaction is done by bringing the cellulose acetate strip containing the phosphatase band into conta...